Molecular Identification of Captive Snakes from their Shed Skin Using Cyt b & 12s rRNA Markers

Aims: To successfully utilize non-invasive shed skin samples for species identification of captive snake Tamil Nadu and create genetic repository.
 Study Design: The experiment was designed to apply the ammonium acetate method DNA extraction from skin. Two mitochondrial markers were used ascertain species.
 Place Duration Study: Advanced Institute Wildlife Conservation (AIWC), Forest Department, Vandalur, Chennai, Nadu. collected between May October 2019 Arignar Anna Zoological Park, Guindy National Amirthi Vellore, Nadu.
 Methodology: We fresh 8 different captivities zoos dried 48 72 hours remove moisture. Independent isolations performed each sample. isolated quantified using Nanodrop Spectrophotometer concentration. PCR amplification regions cytochrome b 12S rRNA agarose gel electrophoresis carried out. products subjected sanger sequencing analyzer.
 Results: concentration all ranged 250 1600 ng/µL average quality ratios A260/280 1.85 A260/230 2.10. Both gene showed specificity in with NCBI BLAST result ranging 99-100%. Phylogenetic trees maximum-likelihood classified closely related under same clade, a bootstrap support 60-100%. Genetic distances 0.148-0.457 region region.
 Conclusion: Shed is often overlooked utilization identification. In this study, snakes extracted amplified Cyt 12s markers. Individual phylogenetic are constructed marker find relatedness one another. This work an initiation repository creation could be effectively employed conservation population studies snakes.